Denizel Diatom İzolasyonu, Tanımlanması ve Besin Maddelerinin Diatom Büyümesi Üzerine Etkisinin İncelenmesi

Sait Çinar, Didem Özçimen, Mete Yılmaz
46 6

Öz


Son yıllarda oldukça dikkat çeken mikroalgler gıda, sağlık, kozmetik, çevre, yakıt alanlarında kullanım potansiyeli bulunan değerli yeni nesil hammadde kaynaklarıdır.Bu çalışmada, hem bulunma miktarı bakımından hem de fonksiyonları bakımından önemli bir mikroalg sınıfı olan diatomların eldesi hedeflenerek, Marmara Denizi Mudanya kıyılarından toplanan su örnekleri üzerinde izolasyon çalışması gerçekleştirilmiş ve elde edilen izolatın moleküler analizi yapılarak Pleurosigma sp. olduğu tespit edilmiştir.

İzole Pleurosigma sp., farklı konsantrasyonlarda azot, fosfor ve silisyum içeren besin solüsyonlarında yetiştirilerek istatistiksel olarak yorumlandığında diatomun hücre yoğunluğunun,  silisyum ve azot ile belirgin şekilde pozitif korelasyon gösterdiği, ancak fosfor ile korelasyonunun seçilen fosfor aralığı için net olmadığı gözlenmiştir.

Pleurosigma sp. ile yapılan karakterizasyon çalışmalarında diatomun önemli miktarda silisyumlu yapılar içerdiği ve kül içeriğinin yüksek olduğu, yağ asidi profilinin literatürde diatomlarda sıklıkla karşılaşılan yağ asitleriyle örtüştüğü görülmüştür.

Anahtar kelimeler


Mikroalg; diatom; Pleurosigma sp.; izolasyon

Tam metin:

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